Detection of Porcine Adulteration in Cosmetic Cream Formulation via TaqMan Probe Real-Time Polymerase Chain Reaction.

Rapid Detection of Porcine Circovirus Type 2 by TaqMan-based Real-time Polymerase Chain Reaction Assays

PCV2 is the primary causative agent of porcine circovirus-associated disease (PCVAD). In this study, a TaqMan-based real-time polymerase chain reaction (PCR) assay targeting ORF2 gene of PCV2 was developed and their sensitivities and specificities were investigated. The results indicated that the standard curve had a wide dynamic range (102-107copies/μL) with a linear correlation (R2) of 0.997 ...

Analysis of pork adulteration in commercial meatballs targeting porcine-specific mitochondrial cytochrome b gene by TaqMan probe real-time polymerase chain reaction.

A test for assessing pork adulteration in meatballs, using TaqMan probe real-time polymerase chain reaction, was developed. The assay combined porcine-specific primers and TaqMan probe for the detection of a 109 bp fragment of porcine cytochrome b gene. Specificity test with 10 ng DNA of eleven different species yielded a threshold cycle (Ct) of 15.5 ± 0.20 for the pork and negative results for...

Detection of Nocardia Asteroides Complex in Clinical Isolates by Real-Time Polymerase Chain Reaction

Background and Aims: Nocardia asteroides complex is the most common cause of infectious diseases due to nocardiosis. Interspecies differentiation of Nocardia genera is essential for prognosis and timely proper treatment, as well as for epidemiological studies. Since each genus has its own antibiotic resistance, precise careful diagnosis is of prime importance. As compared to biochemical and phe...

Detection of Chicken Meat Adulteration in Raw Hamburger Using Polymerase Chain Reaction

Background: Detection of food adulteration is an important issue from aspects of food control and food regulation. This study aimed to detect adulteration of chicken meat in raw hamburger using species specific Polymerase Chain Reaction (PCR). Methods: Raw hamburgers including 42 handmade and 48 industrial samples were collected from 90 restaurants and supermarkets. Following genomic DNA extra...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*